Publications

Regulation of biomolecular condensates by interfacial protein clusters 

Folkmann AW, Putnam A, Lee CF, Seydoux G. Science. 2021 Sep 10;373(6560):1218-1224. doi: 10.1126/science.abg7071. Epub 2021 Sep 9.PMID: 34516789

 

Schmidt H, Putnam A, Rasoloson D, Seydoux G.Elife. 2021 Jun 9;10:e63698. doi: 10.7554/eLife.63698.PMID: 34106046Free PMC article.

 

Cell-free reconstitution of multi-condensate assemblies.

Putnam A, Seydoux G.Methods Enzymol. 2021;646:83-113. doi: 10.1016/bs.mie.2020.07.004. Epub 2020 Oct 20.PMID: 33453934

 

Puromycin reactivity does not accurately localize translation at the subcellular level.

Enam SU, Zinshteyn B, Goldman DH, Cassani M, Livingston NM, Seydoux G, Green R.Elife. 2020 Aug 26;9:e60303. doi: 10.7554/eLife.60303.PMID: 32844748Free PMC article.

 

Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins.

Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G.Elife. 2020 Jan 24;9:e52896. doi: 10.7554/eLife.52896.PMID: 31975687Free PMC article.

 

Author Correction: A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos.

Putnam A, Cassani M, Smith J, Seydoux G.Nat Struct Mol Biol. 2020 Feb;27(2):221. doi: 10.1038/s41594-019-0367-y.PMID: 31896770

 

Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors.

Paix A, Rasoloson D, Folkmann A, Seydoux G.Curr Protoc Mol Biol. 2019 Dec;129(1):e102. doi: 10.1002/cpmb.102.PMID: 31710422Free PMC article.

 

P Granules Protect RNA Interference Genes from Silencing by piRNAs.

Ouyang JPT, Folkmann A, Bernard L, Lee CY, Seroussi U, Charlesworth AG, Claycomb JM, Seydoux G.Dev Cell. 2019 Sep 23;50(6):716-728.e6. doi: 10.1016/j.devcel.2019.07.026. Epub 2019 Aug 8.PMID: 31402283Free PMC article.

 

Phase separation in biology and disease-a symposium report.

Cable J, Brangwynne C, Seydoux G, Cowburn D, Pappu RV, Castañeda CA, Berchowitz LE, Chen Z, Jonikas M, Dernburg A, Mittag T, Fawzi NL.Ann N Y Acad Sci. 2019 Sep;1452(1):3-11. doi: 10.1111/nyas.14126. Epub 2019 Jun 14.PMID: 31199001Free PMC article.

 

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos.

Putnam A, Cassani M, Smith J, Seydoux G.Nat Struct Mol Biol. 2019 Mar;26(3):220-226. doi: 10.1038/s41594-019-0193-2. Epub 2019 Mar 4.PMID: 30833787Free PMC article.

 

Spatial regulation of the polarity kinase PAR-1 by parallel inhibitory mechanisms.

Folkmann AW, Seydoux G.Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116.PMID: 30814118Free PMC article.

 

Dynamics of mRNA entry into stress granules.

Lee CY, Seydoux G.Nat Cell Biol. 2019 Feb;21(2):116-117. doi: 10.1038/s41556-019-0278-5.PMID: 30664788Free PMC article.

 

Phase Separation in Biology and Disease.

Forman-Kay JD, Kriwacki RW, Seydoux G.J Mol Biol. 2018 Nov 2;430(23):4603-4606. doi: 10.1016/j.jmb.2018.09.006. Epub 2018 Sep 11.PMID: 30213727Free PMC article.No abstract available.

 

Single-molecule study reveals the frenetic lives of proteins in gradients.

Folkmann AW, Seydoux G.Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9336-9338. doi: 10.1073/pnas.1812248115. Epub 2018 Sep 4.PMID: 30181287Free PMC article.No abstract available.

 

The P Granules of C. elegans: A Genetic Model for the Study of RNA-Protein Condensates.

Seydoux G.J Mol Biol. 2018 Nov 2;430(23):4702-4710. doi: 10.1016/j.jmb.2018.08.007. Epub 2018 Aug 8.PMID: 30096346Free PMC article.Review.

 

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks.

Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G.Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10745-E10754. doi: 10.1073/pnas.1711979114. Epub 2017 Nov 28.PMID: 29183983Free PMC article.

 

Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B.

Lee CS, Lu T, Seydoux G.Elife. 2017 Nov 7;6:e30201. doi: 10.7554/eLife.30201.PMID: 29111977Free PMC article.

 

Not just Salk.

Greider C, Hopkins N, Steitz J, Amon A, Asai D, Barres B, Bass B, Bassler B, Birgeneau R, Bjorkman P, Botchan M, Brugge J, Cech T, Colwell R, Craig N, deLange T, Eisen M, Gottesman S, Green R, Handelsman J, Kimble J, King MC, Lehmann R, Marder E, Mullins D, O’Shea E, Schmid S, Seydoux G, Spradling A, Storz G, Szostak J, Telesnitsky A, Tilghman S, Tjian R, Vale R, Wolberger C, Zakian V.Science. 2017 Sep 15;357(6356):1105-1106. doi: 10.1126/science.aao6221.PMID: 28912235No abstract available.

 

MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature-Sensitive Mutants via Molecular Inversion Probes.

Mok CA, Au V, Thompson OA, Edgley ML, Gevirtzman L, Yochem J, Lowry J, Memar N, Wallenfang MR, Rasoloson D, Bowerman B, Schnabel R, Seydoux G, Moerman DG, Waterston RH.Genetics. 2017 Oct;207(2):447-463. doi: 10.1534/genetics.117.300179. Epub 2017 Aug 21.PMID: 28827289Free PMC article.

 

Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans.

Paix A, Folkmann A, Seydoux G.Methods. 2017 May 15;121-122:86-93. doi: 10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.PMID: 28392263Free PMC article.

 

Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3.

Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G.Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337.PMID: 27914198Free PMC article.

 

Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.

Housden BE, Muhar M, Gemberling M, Gersbach CA, Stainier DY, Seydoux G, Mohr SE, Zuber J, Perrimon N.Nat Rev Genet. 2017 Jan;18(1):24-40. doi: 10.1038/nrg.2016.118. Epub 2016 Oct 31.PMID: 27795562Free PMC article.Review.

 

Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs.

Paix A, Schmidt H, Seydoux G.Nucleic Acids Res. 2016 Sep 6;44(15):e128. doi: 10.1093/nar/gkw502. Epub 2016 Jun 1.PMID: 27257074Free PMC article.

 

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.

Paix A, Folkmann A, Rasoloson D, Seydoux G.Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.PMID: 26187122Free PMC article.

 

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans.

Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G.Elife. 2014 Dec 23;3:e04591. doi: 10.7554/eLife.04591.PMID: 25535836Free PMC article.

 

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

Chen BC, Legant WR, Wang K, Shao L, Milkie DE, Davidson MW, Janetopoulos C, Wu XS, Hammer JA 3rd, Liu Z, English BP, Mimori-Kiyosue Y, Romero DP, Ritter AT, Lippincott-Schwartz J, Fritz-Laylin L, Mullins RD, Mitchell DM, Bembenek JN, Reymann AC, Böhme R, Grill SW, Wang JT, Seydoux G, Tulu US, Kiehart DP, Betzig E.Science. 2014 Oct 24;346(6208):1257998. doi: 10.1126/science.1257998. Epub 2014 Oct 23.PMID: 25342811Free PMC article.

 

Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.

Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G.Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.PMID: 25249454Free PMC article.

 

P granules.

Wang JT, Seydoux G.Curr Biol. 2014 Jul 21;24(14):R637-R638. doi: 10.1016/j.cub.2014.06.018.PMID: 25050955Free PMC article.No abstract available.

 

Identification of suppressors of mbk-2/DYRK by whole-genome sequencing.

Wang Y, Wang JT, Rasoloson D, Stitzel ML, O’ Connell KF, Smith HE, Seydoux G.G3 (Bethesda). 2014 Feb 19;4(2):231-41. doi: 10.1534/g3.113.009126.PMID: 24347622Free PMC article.

 

The PAR network: redundancy and robustness in a symmetry-breaking system.

Motegi F, Seydoux G.Philos Trans R Soc Lond B Biol Sci. 2013 Sep 23;368(1629):20130010. doi: 10.1098/rstb.2013.0010. Print 2013.PMID: 24062581Free PMC article.Review.

 

‘Goldilocks’ suppressor screen identifies web of polarity regulators.

Seydoux G.Nat Cell Biol. 2013 Jan;15(1):9-10. doi: 10.1038/ncb2666.PMID: 23263368

 

The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells.

Voronina E, Paix A, Seydoux G.Development. 2012 Oct;139(20):3732-40. doi: 10.1242/dev.083980.PMID: 22991439Free PMC article.

 

Germ cell specification.

Wang JT, Seydoux G.Adv Exp Med Biol. 2013;757:17-39. doi: 10.1007/978-1-4614-4015-4_2.PMID: 22872473Free PMC article.Review.

 

Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes.

Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G.Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354.PMID: 21983565Free PMC article.

 

Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle.

Griffin EE, Odde DJ, Seydoux G.Cell. 2011 Sep 16;146(6):955-68. doi: 10.1016/j.cell.2011.08.012.PMID: 21925318Free PMC article.

 

RNA granules in germ cells.

Voronina E, Seydoux G, Sassone-Corsi P, Nagamori I.Cold Spring Harb Perspect Biol. 2011 Dec 1;3(12):a002774. doi: 10.1101/cshperspect.a002774.PMID: 21768607Free PMC article.Review.

 

Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans.

Gallo CM, Wang JT, Motegi F, Seydoux G.Science. 2010 Dec 17;330(6011):1685-9. doi: 10.1126/science.1193697. Epub 2010 Dec 2.PMID: 21127218Free PMC article.

 

The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.

Merritt C, Seydoux G.Development. 2010 Jun;137(11):1787-98. doi: 10.1242/dev.050799. Epub 2010 Apr 28.PMID: 20431119Free PMC article.

 

Symmetry breaking and polarization of the C. elegans zygote by the polarity protein PAR-2.

Zonies S, Motegi F, Hao Y, Seydoux G.Development. 2010 May;137(10):1669-77. doi: 10.1242/dev.045823. Epub 2010 Apr 14.PMID: 20392744Free PMC article.

 

The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules.

Voronina E, Seydoux G.Development. 2010 May;137(9):1441-50. doi: 10.1242/dev.047654. Epub 2010 Mar 24.PMID: 20335358Free PMC article.

 

Transgenic solutions for the germline.

Merritt C, Seydoux G.WormBook. 2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1.PMID: 20169625Free PMC article.Review.No abstract available.

 

Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition.

Cheng KC, Klancer R, Singson A, Seydoux G.Cell. 2009 Oct 30;139(3):560-72. doi: 10.1016/j.cell.2009.08.047.PMID: 19879842Free PMC article.

 

Less is more: specification of the germline by transcriptional repression.

Nakamura A, Seydoux G.Development. 2008 Dec;135(23):3817-27. doi: 10.1242/dev.022434.PMID: 18997110Review.

 

3′ UTRs are the primary regulators of gene expression in the C. elegans germline.

Merritt C, Rasoloson D, Ko D, Seydoux G.Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.PMID: 18818082Free PMC article.

 

Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos.

Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G.Dev Biol. 2008 Nov 1;323(1):76-87. doi: 10.1016/j.ydbio.2008.07.008. Epub 2008 Jul 16.PMID: 18692039Free article.

 

Inhibition of transcription by the Caenorhabditis elegans germline protein PIE-1: genetic evidence for distinct mechanisms targeting initiation and elongation.

Ghosh D, Seydoux G.Genetics. 2008 Jan;178(1):235-43. doi: 10.1534/genetics.107.083212.PMID: 18202370Free PMC article.

 

Conserved regulation of MAP kinase expression by PUF RNA-binding proteins.

Lee MH, Hook B, Pan G, Kershner AM, Merritt C, Seydoux G, Thomson JA, Wickens M, Kimble J.PLoS Genet. 2007 Dec 28;3(12):e233. doi: 10.1371/journal.pgen.0030233.PMID: 18166083Free PMC article.

 

Revisiting the role of microtubules in C. elegans polarity.

Motegi F, Seydoux G.J Cell Biol. 2007 Nov 5;179(3):367-9. doi: 10.1083/jcb.200710062.PMID: 17984317Free PMC article.

 

Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition.

Stitzel ML, Cheng KC, Seydoux G.Curr Biol. 2007 Sep 18;17(18):1545-54. doi: 10.1016/j.cub.2007.08.049.PMID: 17869113Free article.

 

Regulation of the oocyte-to-zygote transition.

Stitzel ML, Seydoux G.Science. 2007 Apr 20;316(5823):407-8. doi: 10.1126/science.1138236.PMID: 17446393Review.

 

Pathway to totipotency: lessons from germ cells.

Seydoux G, Braun RE.Cell. 2006 Dec 1;127(5):891-904. doi: 10.1016/j.cell.2006.11.016.PMID: 17129777Free article.Review.

 

Toti “potent” repressors.

Gallo CM, Seydoux G.Bioessays. 2006 Sep;28(9):865-7. doi: 10.1002/bies.20454.PMID: 16937341

 

The 2006 Genetics Society of America Medal. Victor Ambros.

Seydoux G.Genetics. 2006 Feb;172(2):721-2. doi: 10.1534/genetics.104.17222.PMID: 16505214Free PMC article.No abstract available.

 

Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline.

D’Agostino I, Merritt C, Chen PL, Seydoux G, Subramaniam K.Dev Biol. 2006 Apr 1;292(1):244-52. doi: 10.1016/j.ydbio.2005.11.046. Epub 2006 Feb 24.PMID: 16499902Free article.

 

Stabilization of cell polarity by the C. elegans RING protein PAR-2.

Hao Y, Boyd L, Seydoux G.Dev Cell. 2006 Feb;10(2):199-208. doi: 10.1016/j.devcel.2005.12.015.PMID: 16459299Free PMC article.

 

The C. elegans DYRK Kinase MBK-2 Marks Oocyte Proteins for Degradation in Response to Meiotic Maturation.

Stitzel ML, Pellettieri J, Seydoux G.Curr Biol. 2006 Jan 10;16(1):56-62. doi: 10.1016/j.cub.2005.11.063. Epub 2005 Dec 15.PMID: 16338136Free article.

 

Surfing the actomyosin wave: polarization of the C. elegans zygote.

Seydoux G.Dev Cell. 2004 Sep;7(3):285-6. doi: 10.1016/j.devcel.2004.08.016.PMID: 15363402Free article.

 

A clean start: degradation of maternal proteins at the oocyte-to-embryo transition.

DeRenzo C, Seydoux G.Trends Cell Biol. 2004 Aug;14(8):420-6. doi: 10.1016/j.tcb.2004.07.005.PMID: 15308208Review.

 

Coordinate activation of maternal protein degradation during the egg-to-embryo transition in C. elegans.

Pellettieri J, Reinke V, Kim SK, Seydoux G.Dev Cell. 2003 Sep;5(3):451-62. doi: 10.1016/s1534-5807(03)00231-4.PMID: 12967564Free article.

 

Exclusion of germ plasm proteins from somatic lineages by cullin-dependent degradation.

DeRenzo C, Reese KJ, Seydoux G.Nature. 2003 Aug 7;424(6949):685-9. doi: 10.1038/nature01887. Epub 2003 Jul 23.PMID: 12894212Free PMC article.

 

Polarization of the C. elegans zygote proceeds via distinct establishment and maintenance phases.

Cuenca AA, Schetter A, Aceto D, Kemphues K, Seydoux G.Development. 2003 Apr;130(7):1255-65. doi: 10.1242/dev.00284.PMID: 12588843Free PMC article.

 

Dedifferentiation of primary spermatocytes into germ cell tumors in C. elegans lacking the pumilio-like protein PUF-8.

Subramaniam K, Seydoux G.Curr Biol. 2003 Jan 21;13(2):134-9. doi: 10.1016/s0960-9822(03)00005-8.PMID: 12546787Free article.

 

Anterior-posterior polarity in C. elegans and Drosophila–PARallels and differences.

Pellettieri J, Seydoux G.Science. 2002 Dec 6;298(5600):1946-50. doi: 10.1126/science.1072162.PMID: 12471246Review.

 

cdk-7 Is required for mRNA transcription and cell cycle progression in Caenorhabditis elegans embryos.

Wallenfang MR, Seydoux G.Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5527-32. doi: 10.1073/pnas.082618399.PMID: 11960010Free PMC article.